Spectral phasor imaging on a commercial confocal microscope without a spectral detector

Abstract Spectral imaging is a fluorescence microscopy technique with several applications, including imaging of environment-sensitive probes, spectral unmixing and identification of fluorescent species. In confocal microscopes not equipped with a spectral detection unit, spectral images can be obta...

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Autores principales:	Elisa Longo, Angelita Costantino, Alessio Andreoni, Paolo Bosco, Anna P. Privitera, Silvia Scalisi, Leonel Malacrida, Massimo Gulisano, Luca Lanzanò
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Nature Portfolio 2025-08-01
Colección:	Scientific Reports
Materias:	Spectral phasor Confocal microscope Environment-sensitive probes ACDAN Nile red Fluorescent proteins
Acceso en línea:	https://doi.org/10.1038/s41598-025-15637-x
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