Phasor analysis of RGB camera data enables fluorescence microscopy unmixing and brightfield segmentation in a commercial microscope

Spectral information plays a crucial role in biological imaging, yet conventional epifluorescence and histological techniques often rely on RGB image acquisition, limiting the resolution of spectrally overlapping components. Here, we present a phasor-based spectral analysis framework adapted for RGB...

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Hlavní autoři:	Bruno Schuty, María José García, Satya Khuon, Leonel Malacrida
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2026-06-01
Edice:	Sensing and Bio-Sensing Research
Témata:	Phasor analysis Widefield microscopy RGB imaging Histology segmentation Autofluorescence Spectral unmixing
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S2214180426000644
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