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Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simpl...

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Principais autores: Katharine H. D. Crawford, Rachel Eguia, Adam S. Dingens, Andrea N. Loes, Keara D. Malone, Caitlin R. Wolf, Helen Y. Chu, M. Alejandra Tortorici, David Veesler, Michael Murphy, Deleah Pettie, Neil P. King, Alejandro B. Balazs, Jesse D. Bloom
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2020-05-01
Serier:Viruses
Fag:
SARS-CoV-2
COVID-19
coronavirus
neutralization assay
lentiviral pseudotype
Spike
Online adgang:https://www.mdpi.com/1999-4915/12/5/513
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