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Study of ALDH from <i>Thermus thermophilus</i>—Expression, Purification and Characterisation of the Non-Substrate Specific, Thermophilic Enzyme Displaying Both Dehydrogenase and Esterase Activity

Aldehyde dehydrogenases (ALDH), found in all kingdoms of life, form a superfamily of enzymes that primarily catalyse the oxidation of aldehydes to form carboxylic acid products, while utilising the cofactor NAD(P)<sup>+</sup>. Some superfamily members can also act as esterases using <i>p</i>-nitroph...

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Principais autores: Kim Shortall, Edel Durack, Edmond Magner, Tewfik Soulimane
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2021-12-01
coleção:Cells
Assuntos:
aldehyde dehydrogenase
heat treatment purification
substrate specificity
esterase activity
biochemical characterisation
thermophilic enzyme
Acesso em linha:https://www.mdpi.com/2073-4409/10/12/3535
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