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Enhancement of Protein–Protein Interactions by Destabilizing Mutations Revealed by HDX-MS

Enhancing protein–protein interactions is a key therapeutic strategy to ensure effective protein function in terms of pharmacokinetics and pharmacodynamics and can be accomplished with methods like directed evolution or rationale design. Previously, two papers suggested the possible enhancement of p...

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Principais autores: Yoshitomo Hamuro, Anthony Armstrong, Jeffrey Branson, Sheng-Jiun Wu, Richard Y.-C. Huang, Steven Jacobs
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2025-08-01
Serier:Biomolecules
Fag:
backbone amide
destabilization
hydrogen/deuterium exchange
mass spectrometry
mutation
protein–protein interaction
Online adgang:https://www.mdpi.com/2218-273X/15/8/1201
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