Construction and application of an inducible CRISPR-Cpf1/dsDNA dual-plasmid gene editing system in Corynebacterium glutamicum

Aiming at the problems of low efficiency of gene editing and time-consuming operation in Corynebacterium glutamicum, the Cpf1 was expressed using the inducible promoter PrpR-PrpD2, the recombination and cleavage events were separated, and a high-efficiency inducible CRISPR- Cpf1/dsDNA dual-plasmid g...

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Autor principal:	WANG Ting, SHI Jiabao, GAO Yin, CHEN Ning
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Editorial Department of China Brewing 2025-08-01
coleção:	Zhongguo niangzao
Assuntos:	corynebacterium glutamicum|crispr-cpf1 gene editing system|prpr-prpd2 inducible promoter|l-serine
Acesso em linha:	https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-8-35.pdf
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