Application of the endogenous CRISPR-Cas type I-D system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocaldarius

CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems are widely distributed among bacteria and archaea. In this study, we demonstrate the successful utilization of the type I-D CRISPR-Cas system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocalda...

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Principais autores:	Jan Bost, Alejandra Recalde, Bianca Waßmer, Alexander Wagner, Bettina Siebers, Sonja-Verena Albers
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2023-10-01
coleção:	Frontiers in Microbiology
Assuntos:	archaea genetic tools deletion mutant genetic engineering type I-D CRISPR system protospacer adjacent motif
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254891/full
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