Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in <it>E. coli</it>
<p>Abstract</p> <p>Background</p> <p>Biotin is an essential enzyme cofactor that acts as a CO<sub>2 </sub>carrier in carboxylation and decarboxylation reactions. The <it>E. coli </it>genome encodes a biosynthetic pathway that produces biotin from pimeloyl-CoA in four enzymatic steps. The final step,...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
BMC
2010-10-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/10/73 |
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