Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins
ADP-ribose binding macro domains facilitate the enrichment and detection of cellular ADP-ribosylation. Here, the authors generate an engineered macro domain with increased ADP-ribose affinity, improving the identification of ADP-ribosylated proteins by proteomics, western blot and immunofluorescence...
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| Principais autores: | , , , , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Portfolio
2020-10-01
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| coleção: | Nature Communications |
| Acesso em linha: | https://doi.org/10.1038/s41467-020-18981-w |
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