Protocol for live-cell Förster resonance energy transfer imaging to reveal the bistable insulin response of single C2C12-derived myotubes
Summary: Based on our hypothesis that myotubes exhibit a bistable response to insulin, here we present a protocol for finely measuring Akt phosphorylation in single myotubes under insulin stimulation. We describe steps to stably express a Förster resonance energy transfer (FRET)-based Akt biosensor...
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| Principais autores: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Elsevier
2024-06-01
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| Serija: | STAR Protocols |
| Teme: | |
| Online dostop: | http://www.sciencedirect.com/science/article/pii/S2666166724002740 |
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