Protocol for live-cell Förster resonance energy transfer imaging to reveal the bistable insulin response of single C2C12-derived myotubes

Summary: Based on our hypothesis that myotubes exhibit a bistable response to insulin, here we present a protocol for finely measuring Akt phosphorylation in single myotubes under insulin stimulation. We describe steps to stably express a Förster resonance energy transfer (FRET)-based Akt biosensor...

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Bibliographic Details

Main Authors:	Javed Akhtar, Muhammad Imran, Guanyu Wang
Format:	Artigo
Language:	Inglês
Published:	Elsevier 2024-06-01
Series:	STAR Protocols
Subjects:	Biophysics Cell Biology Single Cell Signal Transduction Biotechnology and bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724002740
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