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Comparison of Three RT-PCR Based Methods for Relative Quantification of mRNA

Comparison of three RT-PCR based methods: semi-quantitative, competitive and real--time RT-PCR for relative quantification of mRNA is presented. Aminopeptidase N expressed on human promyeloid HL-60 cell line, at basal and activated state, served as a model for comparison. HL-60 cells were stimulated...

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Hoofdauteurs: Andreja Ambriović-Ristov, Sanja Kapitanović, Tamara Čačev, Jelka Gabrilovac, Davorka Breljak
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: University of Zagreb Faculty of Food Technology and Biotechnology 2005-01-01
Reeks:Food Technology and Biotechnology
Onderwerpen:
aminopeptidase N
APN
HL-60
RT-PCR
real-time PCR
competitive PCR
Online toegang:http://hrcak.srce.hr/file/162952
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