Genotyping single point mutations in rd1 and rd8 mice using melting curve analysis of qPCR fragments

Abstract PCR is tolerant to single nucleotide mismatches. Therefore, genotyping of point mutations by PCR requires special conditions for the amplification of allele-specific PCR fragments. MS-PCR (mutagenically separated PCR) is an improved version of ARMS (amplification refractory mutation system)...

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Autors principals:	Melanie E. Schwämmle, Felicitas Bucher, Günther Schlunck, Gottfried Martin
Format:	Artigo
Idioma:	Inglês
Publicat:	Nature Portfolio 2024-08-01
Col·lecció:	Scientific Reports
Matèries:	ARMS PCR Genotyping Single nucleotide variant
Accés en línia:	https://doi.org/10.1038/s41598-024-70949-8
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