FAST, a method based on split-GFP for the detection in solution of proteins synthesized in cell-free expression systems

Abstract Cell-free protein synthesis (CFPS) systems offer a versatile platform for a wide range of applications. However, the traditional methods for detecting proteins synthesized in CFPS, such as radioactive labeling, fluorescent tagging, or electrophoretic separation, may be impractical, due to e...

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Hlavní autoři:	Thuy Duong Pham, Chiara Poletti, Therese Manuela Nloh Tientcheu, Massimiliano Cuccioloni, Roberto Spurio, Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Nature Portfolio 2024-04-01
Edice:	Scientific Reports
Témata:	Split-GFP Protein synthesis CFPS Fluorescence CspA
On-line přístup:	https://doi.org/10.1038/s41598-024-58588-5
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