Protocol for the recombinant expression and purification of the LSAM domain of human legumain in E. coli

Summary: Expressing disulfide-rich proteins in E. coli is challenging due to incorrect bond formation. Here, we present a protocol for expressing the PC1pro-LSAM fusion protein in E. coli using the PC1 prodomain as a fusion tag and the legumain stabilization and activity modulation (LSAM) domain as...

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Hauptverfasser:	Sven O. Dahms, Alexander C. Wieland, Hans Brandstetter, Elfriede Dall
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2025-09-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Protein Biochemistry Protein expression and purification Biotechnology and bioengineering
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166725003971
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