Protocol for the recombinant expression and purification of the LSAM domain of human legumain in E. coli
Summary: Expressing disulfide-rich proteins in E. coli is challenging due to incorrect bond formation. Here, we present a protocol for expressing the PC1pro-LSAM fusion protein in E. coli using the PC1 prodomain as a fusion tag and the legumain stabilization and activity modulation (LSAM) domain as...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2025-09-01
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| Col·lecció: | STAR Protocols |
| Matèries: | |
| Accés en línia: | http://www.sciencedirect.com/science/article/pii/S2666166725003971 |
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