Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules
Two-dimensional (2D) agarose gel electrophoresis is the method of choice to analyze DNA topology. The possibility to use E. coli strains with different genetic backgrounds in combination with nicking enzymes and different concentrations of norfloxacin improves the resolution of 2D gels to study the...
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| Principais autores: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Bio-protocol LLC
2024-05-01
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| Serier: | Bio-Protocol |
| Online adgang: | https://bio-protocol.org/en/bpdetail?id=4983&type=0 |
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