Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function
We have developed a technique for expanding primary cultures of human tracheal epithelium while minimizing loss of differentiated structure and function. Cells were seeded at 2 × 104 cells/cm2 into T75 flasks and trypsinized when approximately 80% confluent. The dispersed cells were then passaged at...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Taylor & Francis Group
2005-08-01
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| Серии: | BioTechniques |
| Online-ссылка: | https://www.future-science.com/doi/10.2144/05392RR02 |
| Метки: |
Нет меток, Требуется 1-ая метка записи!
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