Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function
We have developed a technique for expanding primary cultures of human tracheal epithelium while minimizing loss of differentiated structure and function. Cells were seeded at 2 × 104 cells/cm2 into T75 flasks and trypsinized when approximately 80% confluent. The dispersed cells were then passaged at...
Spremljeno u:
| Glavni autori: | , , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Taylor & Francis Group
2005-08-01
|
| Serija: | BioTechniques |
| Online pristup: | https://www.future-science.com/doi/10.2144/05392RR02 |
| Oznake: |
Bez oznaka, Budi prvi tko označuje ovaj zapis!
|