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Protocol to detect nucleotide-protein interaction in vitro using a non-radioactive competitive electrophoretic mobility shift assay

Summary: Electrophoretic mobility shift assay (EMSA) is a classical and popular approach for DNA/RNA protein-binding affinity detection in vitro. This protocol describes a competitive EMSA assay using digoxigenin (DIG)-labeled probe, which solves the safety issues and limitations attributed to the s...

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Principais autores: Fang Wang, Ting Yao, Wen Yang, Pan Wu, Yutao Liu, Bin Yang
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2022-12-01
Serier:STAR Protocols
Fag:
Microbiology
Molecular biology
Molecular/Chemical probes
Protein biochemistry
Protein expression and purification
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166722006104
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