Annealing and purification of fluorescently labeled DNA substrates for in vitro assays

Summary: We present a protocol to generate high-quality fluorescently labeled DNA substrates that can be used for biochemical assays, including DNA-binding and nuclease activity assays. We describe polyacrylamide-gel-electrophoresis-based purification of DNA oligonucleotides, followed by annealing t...

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Détails bibliographiques

Auteurs principaux:	Ying Wah Elizabeth Tse, Hwa Young Yun, Haley Doris Myskiw Wyatt
Format:	Artigo
Langue:	Inglês
Publié:	Elsevier 2023-03-01
Collection:	STAR Protocols
Sujets:	Molecular Biology Molecular/Chemical Probes Protein Biochemistry
Accès en ligne:	http://www.sciencedirect.com/science/article/pii/S2666166723000862
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