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Protocol to screen chemicals to enhance homology-directed repair in CRISPR-Cas9 gene editing

Summary: CRISPR-Cas9-based gene editing via homology-directed repair (HDR) enables precise modifications, though its efficiency is limited by the prevalence of non-homologous end joining (NHEJ). Here, we present a protocol for enhancing HDR efficiency by identifying chemicals using high-throughput s...

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Bibliografische gegevens
Hoofdauteurs: Min Ju Jo, Hye Jin Nam
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2025-09-01
Reeks:STAR Protocols
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Online toegang:http://www.sciencedirect.com/science/article/pii/S2666166725003028
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