Delayed gramicidin delivery through an intra-pipette capillary facilitates perforated patch recordings

The gramicidin-perforated patch-clamp technique is indispensable for recording neuronal activities without changing the intracellular Cl− concentration. Conventionally, gramicidin contained in the pipette fluid is delivered to the cell membrane by passive diffusion. Gramicidin deposited on the pipet...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Kosuke Kazashi, Kakeru Miura, Sota Ueda, Kazuhito Utsunomiya, Mari Kiriyama, Motoki Yagisawa, Masayuki Ikeda, Masanobu Kano, Toshihide Tabata
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2024-10-01
Schriftenreihe:	Neuroscience Research
Schlagworte:	Chloride Gramicidin Perforated-patch Patch-clamp Neuron Seal resistance
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S0168010224000646
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