Multiplex immunofluorescence-guided laser capture microdissection for spatial transcriptomics of metastatic melanoma tissues

Summary: We describe a pipeline for optimized and streamlined multiplexed immunofluorescence-guided laser capture microdissection allowing the harvest of individual cells based on their phenotype and tissue localization for transcriptomic analysis with next-generation RNA sequencing. Here, we analyz...

ver descrição completa

Detalhes bibliográficos

Principais autores:	Jan Martinek, Te-Chia Wu, Lili Sun, Jianan Lin, Kyung In Kim, Florentina Marches, Paul Robson, Joshy George, Karolina Palucka
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2022-12-01
Colecção:	STAR Protocols
Assuntos:	Cell isolation Single cell Cancer RNAseq Immunology Microscopy
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166722005780
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!