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Multiplex immunofluorescence-guided laser capture microdissection for spatial transcriptomics of metastatic melanoma tissues

Summary: We describe a pipeline for optimized and streamlined multiplexed immunofluorescence-guided laser capture microdissection allowing the harvest of individual cells based on their phenotype and tissue localization for transcriptomic analysis with next-generation RNA sequencing. Here, we analyz...

Whakaahuatanga katoa

I tiakina i:
Ngā kaituhi matua: Jan Martinek, Te-Chia Wu, Lili Sun, Jianan Lin, Kyung In Kim, Florentina Marches, Paul Robson, Joshy George, Karolina Palucka
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Elsevier 2022-12-01
Rangatū:STAR Protocols
Ngā marau:
Cell isolation
Single cell
Cancer
RNAseq
Immunology
Microscopy
Urunga tuihono:http://www.sciencedirect.com/science/article/pii/S2666166722005780
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