Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
Abstract Background The methylotrophic yeast Pichia pastoris is considered as an ideal host for the production of recombinant proteins and chemicals. However, low homologous recombination (HR) efficiency hinders its precise and extensive genetic manipulation. To enhance the homology-directed repair...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BMC
2022-09-01
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| Serie: | Microbial Cell Factories |
| Soggetti: | |
| Accesso online: | https://doi.org/10.1186/s12934-022-01908-z |
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