Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

Abstract Background The methylotrophic yeast Pichia pastoris is considered as an ideal host for the production of recombinant proteins and chemicals. However, low homologous recombination (HR) efficiency hinders its precise and extensive genetic manipulation. To enhance the homology-directed repair...

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Detalhes bibliográficos

Principais autores:	Kun Zhang, Xingpeng Duan, Peng Cai, Linhui Gao, Xiaoyan Wu, Lun Yao, Yongjin J. Zhou
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2022-09-01
coleção:	Microbial Cell Factories
Assuntos:	Pichia pastoris CRISPR/Cas9 Gene editing Homology-directed repair Metabolic engineering
Acesso em linha:	https://doi.org/10.1186/s12934-022-01908-z
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