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Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification

Single-molecule fluorescent in situ hybridization (smFISH) has emerged as a powerful technique that allows one to localize and quantify the absolute number of mRNAs in single cells. In combination with immunofluorescence (IF), smFISH can be used to correlate the expression of an mRNA and a protein o...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Anna Maekiniemi, Robert H. Singer, Evelina Tutucci
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2020-06-01
Schriftenreihe:Data in Brief
Schlagworte:
Single molecule mRNA FISH
smFISH
Immunofluorescence
smFISH-IF
Single cell
Imaging analysis
Online-Zugang:http://www.sciencedirect.com/science/article/pii/S2352340920304054
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