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3D exploration of gene expression in chicken embryos through combined RNA fluorescence in situ hybridization, immunofluorescence, and clearing

Abstract Background Fine characterization of gene expression patterns is crucial to understand many aspects of embryonic development. The chicken embryo is a well-established and valuable animal model for developmental biology. The period spanning from the third to sixth embryonic days (E3 to E6) is...

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Principais autores: Maëlys André, Sarah Dinvaut, Valérie Castellani, Julien Falk
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2024-06-01
Serier:BMC Biology
Fag:
RNA fluorescence in situ hybridization (HCR RNA-FISH)
Immunofluorescence (IF)
Tissue clearing
ECi
Whole mount chicken embryos
Development
Online adgang:https://doi.org/10.1186/s12915-024-01922-0
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