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Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination

Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a Pseudomonas-specific PEHR system. However, when using the PEHR system for Pseudomonas genome editing, false positives can be a problem. In this study, we combined a...

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Principais autores: Wentao Zheng, Yandong Xia, Xue Wang, Shiqing Gao, Diao Zhou, Jun Fu, Ruijuan Li, Jia Yin
Format: Artigo
Sprog:Inglês
Udgivet: KeAi Communications Co., Ltd. 2022-12-01
Serier:Engineering Microbiology
Fag:
Phage-encoded homologous recombination
Pseudomonas
Cascade-Cas3 system
Genome engineering
Online adgang:http://www.sciencedirect.com/science/article/pii/S2667370322000376
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