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Automated solid-phase subcloning based on beads brought into proximity by magnetic force.

In the fields of proteomics, metabolic engineering and synthetic biology there is a need for high-throughput and reliable cloning methods to facilitate construction of expression vectors and genetic pathways. Here, we describe a new approach for solid-phase cloning in which both the vector and the g...

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Detalhes bibliográficos
Principais autores: Elton P Hudson, Andrej Nikoshkov, Mathias Uhlen, Johan Rockberg
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science (PLoS) 2012-01-01
Colecção:PLoS ONE
Acesso em linha:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22624028/?tool=EBI
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