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Single‐EV Analyses Require Rigorous Antibody Qualification: PD‐L1 Profiling in Cell Models and Patient Plasma

ABSTRACT Imaging flow cytometry (IFCM) has emerged as a powerful method for high‐throughput phenotyping of small extracellular vesicles (sEVs) at single‐vesicle resolution. However, the reliability and sensitivity of IFCM critically depend on the performance of the antibodies used. In this study, we...

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Principais autores: Tobias Tertel, Fabiola Nardi Bauer, Oumaima Stambouli, Sabine Kasimir‐Bauer, Corinna Keup, Stephan Lang, Ali Sak, Martin Stuschke, Tienush Rassaf, Martin Schuler, Stefan Kasper‐Virchow, Bernd Giebel, Alexander Schramm
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley 2026-05-01
coleção:Journal of Extracellular Biology
Assuntos:
antibody validation
cancer plasma
extracellular vesicles
imaging flow cytometry
PD‐L1
single‐vesicle analysis
Acesso em linha:https://doi.org/10.1002/jex2.70144
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