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Single-molecule Analysis of DNA Replication Dynamics in Budding Yeast and Human Cells by DNA Combing

The DNA combing method allows the analysis of DNA replication at the level of individual DNA molecules stretched along silane-coated glass coverslips. Before DNA extraction, ongoing DNA synthesis is labeled with halogenated analogues of thymidine. Replication tracks are visualized by immunofluoresce...

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Bibliografiske detaljer
Principais autores: Hélène Tourrière, Julie Saksouk, Armelle Lengronne, Philippe Pasero
Format: Artigo
Sprog:Inglês
Udgivet: Bio-protocol LLC 2017-06-01
Serier:Bio-Protocol
Online adgang:https://bio-protocol.org/en/bpdetail?id=2305&type=0
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