Protocol to dissociate healthy and infected murine- and hamster-derived lung tissue for single-cell transcriptome analysis

Summary: In infectious disease research, single-cell RNA sequencing allows dissection of host-pathogen interactions. As a prerequisite, we provide a protocol to transform solid and complex organs such as lungs into representative diverse, viable single-cell suspensions. Our protocol describes perfor...

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Hlavní autoři:	Peter Pennitz, Cengiz Goekeri, Jakob Trimpert, Emanuel Wyler, Aileen Ebenig, Chantal Weissfuss, Michael D. Mühlebach, Martin Witzenrath, Geraldine Nouailles
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2023-03-01
Edice:	STAR Protocols
Témata:	Cell Biology Cell isolation Single Cell
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S2666166722008371
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