In vitro Transcription (IVT) and tRNA Binding Assay
This protocol describes the coupling of (i) “live” in vitro RNA transcription with (ii) binding by a radiolabeled, pre-formed tRNA followed by native gel electrophoresis and phosphorimager scan to visualize the complex. The necessity arose from the stable structure that one RNA forms in the absence...
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| Hlavní autor: | |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Bio-protocol LLC
2014-09-01
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| Edice: | Bio-Protocol |
| On-line přístup: | https://bio-protocol.org/en/bpdetail?id=1234&type=0 |
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