The genomic copy number determination of improved Cre in transgenic mice using standards derived from megaprimer PCR constructed tandem repeats

Determination of the genomic copy number of Cre transgene is critical for the establishment of transgenic animal models. We demonstrate a real-time quantitative polymerase chain reaction (RT-qPCR) based method for genomic copy number determination of improved Cre (iCre), which was tested in a transg...

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Auteurs principaux:	Ge Diao, Min Tian, Runbo Li, Jie Huang, Jian Han, Jianxin Guo
Format:	Artigo
Langue:	Inglês
Publié:	Taylor & Francis Group 2024-12-01
Collection:	All Life
Sujets:	megaprimer pcr tandem repeat cre transgene genomic copy number real-time quantitative pcr
Accès en ligne:	http://dx.doi.org/10.1080/26895293.2024.2316078
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