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The genomic copy number determination of improved Cre in transgenic mice using standards derived from megaprimer PCR constructed tandem repeats

Determination of the genomic copy number of Cre transgene is critical for the establishment of transgenic animal models. We demonstrate a real-time quantitative polymerase chain reaction (RT-qPCR) based method for genomic copy number determination of improved Cre (iCre), which was tested in a transg...

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Principais autores: Ge Diao, Min Tian, Runbo Li, Jie Huang, Jian Han, Jianxin Guo
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis Group 2024-12-01
Serier:All Life
Fag:
megaprimer pcr
tandem repeat
cre transgene
genomic copy number
real-time quantitative pcr
Online adgang:http://dx.doi.org/10.1080/26895293.2024.2316078
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