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An improved intracellular staining protocol for efficient detection of nuclear proteins in YFP-expressing cells

Intracellular staining is a widely used flow cytometry (FCM)-based technique to detect the expression of cytoslio nucleic antigens. However, intracellular staining of cells expressing cytosolic fluorescent protein (FP) markers was proven to be problematic as significant loss of the FP-signal was rou...

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Principais autores: Maria Grupillo, Robert Lakomy, Xuehui Geng, Alexis Styche, William A Rudert, Massimo Trucco, Yong Fan
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis Group 2011-12-01
coleção:BioTechniques
Assuntos:
Intracellular staining
Flow Cytometry
Membrane Permeabilization
YFP
Foxp3
T regulatory cells
Acesso em linha:https://www.future-science.com/doi/10.2144/000113780
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