Refolding of ribonuclease A in vitro and conformational characterization by spectrofluorimetry
Ribonuclease A (RNase A) was employed as a model protein to understand the formation of correct three-dimensional structure in the refolding of recombinant proteins in vitro. Important parameters such as the concentration of urea, dilution fold, redox environment (the concentration of GSH and the ra...
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Zhejiang University Press
2006-09-01
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| Colecção: | 浙江大学学报. 农业与生命科学版 |
| Assuntos: | |
| Acesso em linha: | https://www.academax.com/doi/10.3785/1008-9209.2006.05.0483 |
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