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Refolding of ribonuclease A in vitro and conformational characterization by spectrofluorimetry

Ribonuclease A (RNase A) was employed as a model protein to understand the formation of correct three-dimensional structure in the refolding of recombinant proteins in vitro. Important parameters such as the concentration of urea, dilution fold, redox environment (the concentration of GSH and the ra...

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Bibliografske podrobnosti
Principais autores: TAN Pei-sheng, LUO Man, SHENG Qing, GUAN Yi-xin, YAO Shan-jing
Format: Artigo
Jezik:Inglês
Izdano: Zhejiang University Press 2006-09-01
Serija:浙江大学学报. 农业与生命科学版
Teme:
Online dostop:https://www.academax.com/doi/10.3785/1008-9209.2006.05.0483
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