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Visualizing subcellular changes in the NAD(H) pool size versus redox state using fluorescence lifetime imaging microscopy of NADH

Abstract NADH autofluorescence imaging is a promising approach for visualizing energy metabolism at the single-cell level. However, it is sensitive to the redox ratio and the total NAD(H) amount, which can change independently from each other, for example with aging. Here, we evaluate the potential...

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Autors principals: Angela Song, Nicole Zhao, Diana C. Hilpert, Caroline Perry, Joseph A. Baur, Douglas C. Wallace, Patrick M. Schaefer
Format: Artigo
Idioma:Inglês
Publicat: Nature Portfolio 2024-04-01
Col·lecció:Communications Biology
Accés en línia:https://doi.org/10.1038/s42003-024-06123-7
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