Protocol for genome-wide CRISPR knockout screens of bacterial cytotoxins in HeLa cells

Summary: CRISPR screening is a powerful tool to identify host factors for pathogenic agents including viruses and bacterial toxins. Here, we present a protocol to conduct a genome-scale CRISPR screen on HeLa cells for host factors involved in the toxin action of Clostridioides difficile TcdB4. We de...

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Autori principali:	Qi Yang, Yao Zhou, Liuqing He, Yuanyuan Zhang, Liang Tao
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Elsevier 2022-09-01
Serie:	STAR Protocols
Soggetti:	Sequence analysis Cell Biology Cell culture Sequencing High Throughput Screening Microbiology
Accesso online:	http://www.sciencedirect.com/science/article/pii/S2666166722004750
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