DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos
Summary: Cell-free DNA (cfDNA) in spent embryo culture media (SECM) provides prospects for noninvasive preimplantation genetic testing. Here, we present a post-bisulfite-adapter-tagging (PBAT)-based whole-genome DNA methylation sequencing protocol (SECM-PBAT) for human SECM cfDNA analysis. We descri...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2023-06-01
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| Serie: | STAR Protocols |
| Soggetti: | |
| Accesso online: | http://www.sciencedirect.com/science/article/pii/S2666166723002058 |
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