SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e

Increased-fidelity SpCas9 variants have been developed, but often show proportionally reduced activity. Here the authors characterise the on-target activity and off-target propensity of SuperFi-Cas9 in mammalian cells and also see strongly reduced activity but with high fidelity features.

Bibliografiske detaljer

Principais autores:	Péter István Kulcsár, András Tálas, Zoltán Ligeti, Sarah Laura Krausz, Ervin Welker
Format:	Artigo
Sprog:	Inglês
Udgivet:	Nature Portfolio 2022-11-01
Serier:	Nature Communications
Online adgang:	https://doi.org/10.1038/s41467-022-34527-8
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