Protocol for quantum dot-based cell counting and immunostaining of pulmonary arterial cells from patients with pulmonary arterial hypertension

Summary: Currently, there is no protocol for growing and culturing primary pulmonary arterial cells (PACs) available from the Pulmonary Hypertension Breakthrough Initiative (PHBI). Here, we present a protocol for cultivating and maintaining three major PACs collected from patients with pulmonary art...

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Autori principali:	Sakib M. Moinuddin, Md Ibrahim, Tanoy Sarkar, Md Shahadat Hossain, Melanie Rose, Fakhrul Ahsan
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Elsevier 2024-12-01
Serie:	STAR Protocols
Soggetti:	Cell culture Cell isolation Microscopy Molecular/Chemical Probes
Accesso online:	http://www.sciencedirect.com/science/article/pii/S2666166724004842
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