Are we throwing away good data? Evaluation of chimera detection algorithms on long-read amplicons reveals high false-positive rates across algorithms

Long-read amplicon sequencing has enabled us to return to full-length DNA barcodes, which benefit from the higher taxonomic resolution in metabarcoding-based biodiversity studies. However, chimeric sequences (artificial constructs formed when incomplete amplicons fuse during polymerase chain reactio...

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Detalhes bibliográficos

Principais autores:	Ali Hakimzadeh, Vladimir Mikryukov, Martin Metsoja, Leho Tedersoo, Sten Anslan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	PeerJ Inc. 2025-12-01
coleção:	PeerJ
Assuntos:	Long-read sequencing De novo chimera detection Metabarcoding Bioinformatics rRNA Internal transcribed spacer
Acesso em linha:	https://peerj.com/articles/20456.pdf
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