Are we throwing away good data? Evaluation of chimera detection algorithms on long-read amplicons reveals high false-positive rates across algorithms

Long-read amplicon sequencing has enabled us to return to full-length DNA barcodes, which benefit from the higher taxonomic resolution in metabarcoding-based biodiversity studies. However, chimeric sequences (artificial constructs formed when incomplete amplicons fuse during polymerase chain reactio...

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Bibliografische Detailangaben

Hauptverfasser:	Ali Hakimzadeh, Vladimir Mikryukov, Martin Metsoja, Leho Tedersoo, Sten Anslan
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	PeerJ Inc. 2025-12-01
Schriftenreihe:	PeerJ
Schlagworte:	Long-read sequencing De novo chimera detection Metabarcoding Bioinformatics rRNA Internal transcribed spacer
Online-Zugang:	https://peerj.com/articles/20456.pdf
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