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Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast

Summary: Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast cell biology studies. Here, we present a step-by-s...

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Hlavní autoři: Andrew K. Lamb, Santiago M. Di Pietro
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2022-06-01
Edice:STAR Protocols
Témata:
Cell Biology
Cell-based Assays
Microscopy
Model Organisms
Signal Transduction
On-line přístup:http://www.sciencedirect.com/science/article/pii/S2666166722002039
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