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A novel system for stable, high-level expression from the T7 promoter

<p>Abstract</p> <p>Background</p> <p>The most widespread, efficient prokaryotic protein-producing system is one where the T7 phage polymerase recognizes the T7 phage promoter (T7 p/p system). Unfortunately, in this system, target protein expression gradually declines and is often undetectable follow...

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Principais autores: Kesik-Brodacka Malgorzata, Romanik Agnieszka, Mikiewicz-Sygula Diana, Plucienniczak Grazyna, Plucienniczak Andrzej
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2012-08-01
Serier:Microbial Cell Factories
Fag:
T7 expression
Expression cassette
Stable expression from T7 promoter
High expression from T7 promoter
Online adgang:http://www.microbialcellfactories.com/content/11/1/109
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