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Monitoring GPCR conformation with GFP-inspired dyes

Summary: Solvatochromic compounds have emerged as valuable environment-sensitive probes for biological research. Here we used thiol-reactive solvatochromic analogs of the green fluorescent protein (GFP) chromophore to track conformational changes in two proteins, recoverin and the A2A adenosine rece...

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Principais autores: Anatoliy Belousov, Ivan Maslov, Philipp Orekhov, Polina Khorn, Pavel Kuzmichev, Nadezhda Baleeva, Vladislav Motov, Andrey Bogorodskiy, Svetlana Krasnova, Konstantin Mineev, Dmitry Zinchenko, Evgeni Zernii, Valentin Ivanovich, Sergei Permyakov, Johan Hofkens, Jelle Hendrix, Vadim Cherezov, Thomas Gensch, Alexander Mishin, Mikhail Baranov, Alexey Mishin, Valentin Borshchevskiy
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2024-08-01
Series:iScience
Assuntos:
Biochemistry
Structural biology
Biophysics
Acceso en liña:http://www.sciencedirect.com/science/article/pii/S2589004224016912
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