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Enzymatic Synthesis of Highly Fluorescent 8-Azapurine Ribosides Using a Purine Nucleoside Phosphorylase Reverse Reaction: Variable Ribosylation Sites

Various forms of purine-nucleoside phosphorylase (PNP) were used as catalysts of enzymatic ribosylation of selected fluorescent 8-azapurines. It was found that the recombinant calf PNP catalyzes ribosylation of 2,6-diamino-8-azapurine in a phosphate-free medium, with ribose-1-phosphate as ribose don...

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Principais autores: Goran Mikleušević, Alicja Stachelska-Wierzchowska, Beata Wielgus-Kutrowska, Jacek Wierzchowski
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2013-10-01
Serier:Molecules
Fag:
8-azapurines
nucleosides
enzymatic synthesis
fluorescence
purine-nucleoside phosphorylase
Online adgang:http://www.mdpi.com/1420-3049/18/10/12587
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